| 1. | When a pure line variety is grown at close spacing there is a competition effect .
当一个纯系品种在密的植距下生长时有一种竞争效应。
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| 2. | Selection of somatic embryogenesis pure lines of two upland cotton gossypium hirsutum l . cultivars
两个陆地棉体细胞胚胎发生新品系的选育
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| 3. | It is differ both from the method of " circulate line selection " for seed reproduction and from the method of " pure line breeding " for varieties development
该方法既区别于单纯生产原种的“株系循环法” ，也区别于选育新品种的纯系育种法。
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| 4. | Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions
本研究以反义乙烯受体leetr1 ， leetr2基因番茄t _ 0代种子为实验材料，利用gus基因表达研究外源基因的遗传规律，并借助于pcr技术对目的和标记基因的鉴定获得转基因t _ 1代材料。利用gus基因在t1花粉中的表达鉴定获得转基因纯合植株。研究了转基因后代的生长发育模式、对外源乙烯敏感性，以及靶基因的表达特性，初步探明了它们在乙烯受体系统中的功能。
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| 5. | These results demonstrated that merely examining the segregation of reporter gene in pollens is a reliable method that could avoid the laborious testing process of the pure line from transformed plants ; ( 3 ) the transcript levels of leetrl and leetrl were checked by northern blotting analysis
( 3 )两个基因在反义植株里的表达都受到不同程度的抑制。在反义leetr1植株里， leetr1表达被抑制后， leetr2的表达也受到不同程度的抑制。同时，反义leetr2植株里也有相似的结果。
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